īernal V, Carinhas N, Yokomizo AY, Carrondo MJT, Alves PM (2009) Cell density effect in the baculovirus-insect cells system: A quantitative analysis of energetic metabolism. Ĭha HJ, Dalal NG, Pham MQ, Kramer SF, Vakharia VN, Bentley WE (2002) Monitoring foreign protein expression under baculovirus p10 and polh promoters in insect larvae. Xue J, Qiao N, Zhang W, Cheng RL, Zhang XQ, Bao YY, Xu YP, Gu LZ, Han JJ, Zhang CX (2012) Dynamic interactions between Bombyx mori nucleopolyhedrovirus and its host cells revealed by transcriptome analysis. Nobiron I, O’Reilly DR, Olszewski JA (2003) Autographa californica nucleopolyhedrovirus infection of Spodoptera frugiperda cells: a global analysis of host gene regulation during infection, using a differential display approach. Iwanaga M, Shimada T, Kobayashi M, Kang W (2007) Identification of differentially expressed host genes in Bombyx mori nucleopolyhedrovirus infected cells by using subtractive hybridization. Ikeda M, Kobayashi M (1999) Cell-cycle perturbation in Sf9 cells infected with Autographa californica nucleopolyhedrovirus. Rohrmann GF (2013) Baculovirus Molecular Biology, 4th ed National Center for Biotechnology Information: Bethesda (MD), USA, 2019, Available from: Ĭlem RJ (2005) The role of apoptosis in defense against baculovirus infection in insects. Herniou EA, Luque T, Chen X, Vlak JM, Winstanley D, Cory JS, O’Reilly DR (2001) Use of whole genome sequence data to infer baculovirus phylogeny. Jehle JA, Blissard GW, Bonning BC, Cory JS, Herniou EA, Rohrmann GF, Theilmann DA, Vlak JM (2006) On the classification and nomenclature of baculoviruses: a proposal for revision. The present results indicated that BmGP37 is one of the ODV-associated proteins and may have important roles in host mitochondria during BmNPV infection. Furthermore, western blot analysis revealed that BmGP37 is a novel component protein of the occlusion-derived virus (ODV) of BmNPV. Immunofluorescence analysis demonstrated that BmGP37 localized to the host mitochondria during BmNPV infection. ![]() Western blot experiments showed that BmGP37 was expressed at 18 h post-infection and was verified as a mitochondria-associated protein. Furthermore, the BmGP37 antibodies were generated, which could react specifically with BmGP37 in the BmNPV-infected BmN cells. ![]() One mitochondria-associated protein in virus-infected cells was identified as BmGP37 by liquid chromatography-mass spectrometry analysis. Herein, the proteins associated with host mitochondria isolated from BmNPV-infected or mock-infected cells by two-dimensional gel electrophoresis were compared. This study described the involvement of Bombyx mori nucleopolyhedrovirus (BmNPV) GP37 (BmGP37) in host mitochondria. Mitochondria play an essential role in intracellular energy metabolism.
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